Appendix

Appendix 1. Production of Fertilizer at Tanzania Fertilizer Company Plant Tanga 1972-1990 (000 Mt)

Year	S/A	TSP	DAP	NPK	Blends	Total
1972	-	6.6	2.0	6.2	-	14.8
1973	4.7	9.9	-	1.7	-	11.6
1974	15.4	24.9	0.5	18.3	-	59.1
1975	18.0	19.7	-	1.6	-	39.3
1976	18.3	7.2	6.6	4.0	-	36.1
1977	15.7	8.3	-	12.8	-	36.8
1978	16.1	8.2	-	20.1	-	44.4
1979	25.4	4.5	-	8.2	9.1	47.2
1980	19.5	12.3	-	13.9	5.1	50.8
1981	24.9	14.5	-	13.2	14.4	67.0
1982	6.8	2.9	-	-	4.0	13.7
1983	14.8	7.1	-	.5	6.8	29.2
1984	22.6	8.8	-	-	20.2	51.6
1985	15.2	8.2	-	-	18.0	41.4
1986	19.3	3.5	-	-	24.2	47.0
1987	8.8	.5	-	-	10.0	19.3
1988	3.5	-	-	-	3.4	6.9
1989	14.9	3.4	-	-	8.8	27.1

Appendix 2. Analysis of Influent and Effluent, Oljoro Military Camp

Specimen	Inf. 1	Infl. 2	Infl. 3	Holding	Effl.	Effl.	Effl.
Salmonella	-	NSG	NSG	NSG	NSG	NSG	NSG
V. cholera	-	-	-	-	-	-	-
Shigella	NSG	NSG	NSG	NSG	NSG	NSG	NSG
P.E. Coli	-	-	-	-	-	-	-
Entamoeba	+	-	+	+	+	+	-
Ascaris	-	-	+	-	-	-	-
Trichuris	-	-	+	-	-	-	-
Hookworms	-	+	-	-	+	+	-
Schistosoma	-	-	-	-	-	-	-
Taenia	-	-	-	-	-	-	-

Tests were conducted at Nairobi University, March 1993.

NSG = No significant growth (noted)
- = negative
+ = positive
1, 2, 3: Different zones in the main digester

Appendix 3. Pathogens Found at Sampled Sites

Distance from digester bottom	Biogas Extension Service 16 m²	Oljoro Military Camp 32 m²	Livestock Institute, Tengeru 16 m²	Patandi Hospital, Tengeru 30 m²
0.5 m	Ascaris ova	Ascaris ova	negative	negative
1.0 m	negative	Ascaris ova	Ascaris ova	negative
1.5 m	negative	negative	negative	Shigella dysenteriae

Pathogens tested for included salmonellas, shigellas, Vibrio cholerae, Path E.Coli, Entamoeba, Ascaris, Trichuris, Hookworms, Schistosoma, and Taenia.

Tests were carried out at Kenya Medical Research Centre, Department of Microbiology, July 1993.

Appendix 4. Pathogens Found at Sampled Sites

Specimen	BES Biolatrine	Oljoro Military Camp Biolatrine	Livestock Training Institute Biolatrine
Influent	Ascaris ova	Ascaris ova	Ascaris ova
Effluent	negative	negative	negative

Analysis was carried out in Mount Meru Hospital, Arusha, August 1993.

Pathogens tested for included salmonellas, shigellas, Vibrio cholerae, Path. E.Coli, Entamoeba, Ascaris, Hookworms, Schistosoma, and Taenia.

Appendix 5. Pathogens in Vegetables Irrigated with Biolatrine Effluents

Specimen	Enter - virus	V cholera	Path. E. Coli	Shigella	Salmonella	Enteric Parasites
M-onion	-	-ve	-ve	-ve	-ve	-
M-tomato	-	-ve	-ve	-ve	-ve	-
M-cabbage	-	-ve	-ve	-ve	-ve	-
M-carrot	-	-ve	-ve	-ve	-ve	-
B-cabbage	-	-ve	+ve	-ve	-ve	-
B-tomato	-	-ve	-ve	-ve	-ve	-
B-carrot	-	-ve	-ve	-ve	-ve	-
B-onion	-	-ve	+ve	-ve	-ve	-

The sample vegetables were from the test pilot at the Biogas Extension Service Office - Arusha.

Key: -ve = negative
+ve = positive
- = not done
M = Market sample
B = Vegetable fertilized by the biolatrine effluent

Appendix 6. Coliform Bacteria in Vegetables, Bacteria Count per Gram

Vegetable	Brought from Arusha public market	Picked from test garden fed on biolatrine effluent - Biogas Extension Service Office, Arusha
Spinach	more than 2400/g	negative
Lettuce	75/g	11/g
Tomato	negative	negative

The laboratory tests were conducted at and by the Tanzania Bureau of Standards, Dar es Salaam, March 1993.

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Ernest G. Green

Vice Chair

Willie Grace Campbell

Members

Cecil J. Banks
Marion M. Dawson
John F. Hicks, Sr.
George E. Moose

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